development and evaluation of a quadruplex taq man real-time pcr assay for simultaneous detection of clinical isolates of enterococcus faecalis, enterococcus faecium and their vana and vanb genotypes.

Development and evaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes

BACKGROUND AND OBJECTIVES We developed and evaluated the utility of a quadruplex Taqman real-time PCR assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. MATERIALS AND METHODS The specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were ...

Detection and Distribution of various HLAR Gene in Enterococcus faecalis and Enterococcus faecium by Multiplex-PCR

Background: In recent years, three new aminoglycoside resistance genes such as aph (3ʹ)-IIIa and ant (4ʹ)-Ia, that encode for the APH (3ʹ) and ANT (4ʹ) have also been identified. The aim of this study was to come up with a multiplex-PCR procedure for detection of aac(6ʹ)-Ie–aph(2ʹʹ)-Ia, aph(3ʹ)-IIIa, ant(4ʹ)-Ia genes in the Enterococcus spp. clinical isolates. Material and Method: 100 ...

vanA in Enterococcus faecium, Enterococcus faecalis, and Enterococcus casseliflavus detected in French cattle.

The goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in French cattle. Fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. Vancomycin resistance was principally encountered in Enterococcus gallinarum and Enterococcus casseliflavus strains. However, glycopeptide re...

Detection of both vanA & vanB genes in vanA phenotypes of Enterococci by Taq Man RT-PCR

Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.

Susceptibility testing of clinical isolates of Enterococcus faecium and Enterococcus faecalis.

We collected 103 clinical Enterococcus faecium isolates from across Canada, performed standard broth microdilution susceptibility testing, and compared these results with results from the MicroScan Pos MIC Type 6 panel (Baxter Health Care Corp., West Sacramento, Calif.) and the AMS-Vitek Gram-Positive Susceptibility card (Vitek Inc., St. Louis, Mo.). High-level aminoglycoside resistance to gent...

VanB phenotype-vanA genotype Enterococcus faecium with heterogeneous expression